RESEARCH ARTICLE


Diversity of Woody Species and Biomass Carbon Stock in Response to Exclosure Age in Central Dry Lowlands of Ethiopia



Temima Ibrahim1
iD
, Fikrey Tesfay2, *
iD
, Bethel Geremew2
iD

1 Department of Forestry, College of Agriculture, Wollo University, Dessie, Ethiopia
2 Department of Natural Recourse Management, College of Agriculture and Natural Resources Sciences, Debre Birhan University, Debre Birhan, Ethiopia


© 2021 Ibrahim et al.

open-access license: This is an open access article distributed under the terms of the Creative Commons Attribution 4.0 International Public License (CC-BY 4.0), a copy of which is available at: https://creativecommons.org/licenses/by/4.0/legalcode. This license permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

* Address correspondence to this author at the Department of Natural Recourse Management, College of Agriculture and Natural Resources Sciences, Debre Birhan University, Debre Birhan, Ethiopia; E-mail: fikreytesfay@gmail.com


Abstract

Objective:

Empirical evidence on the potential of area exclosure in the restoration of severely degraded lands is crucially important. Thus, a study was conducted to examine the influence of exclosure age on vegetation structure, diversity, and biomass carbon stock in the central dry lowland of Ethiopia.

Methods:

Exclosures of 5, 15, >20 years old, and adjacent open grazing land were selected. Data on vegetation were collected using 20 × 20 m sampling quadrats which were laid along parallel transect lines.

Results:

The result showed that 17 woody species which represent 9 families were recorded at exclosures and open grazing lands. Shannon-Wiener (H') diversity index ranged from 0.74 (open grazing land) to 2.12 (middle age exclosure). Shannon evenness (E) index was higher in the middle age exclosure (0.80). Woody species basal area and tree density significantly (p < 0.05) increased with increasing exclosure age. The Aboveground woody biomass significantly (p < 0.05) varied from 12.60 (open grazing land) to 68.61 Mg ha-1 (middle age exclosure). Similarly, the aboveground biomass (AGB) carbon stocked was significantly (p < 0.05) higher in the middle (32 Mg ha-1) and old age exclosures (31 Mg ha-1).

Conclusion:

This study indicated that exclusion can restore the degraded vegetation and sequester and stock more atmospheric carbon dioxide in the aboveground biomass. Therefore, open degraded grazing land of the lowland areas can be restored into a promising stage through area exclosure land use management.

Keywords: Acacia species, Biomass, Degraded landscape, Grazing land, Woody species, Vegetation structure.



1. INTRODUCTION

Land degradation which is a long lasting decline in the provision capacity of an ecosystem to provide goods and services (Appanah et al., 2015) is widely recognized as a serious global problem, particularly in developing countries. When coupled with climate change effects, it undermines food security (Etter, 2016). The Ethiopian highlands have been degraded and have reached catastrophic levels over the last three centuries (Berisso, 1995; Nyssen et al., 2015). The chief causes of land degradation in the country are population pressure and limited agricultural technologies, inappropriate land use and cultivation into marginal lands, and deforestation (Haile et al., 2006; Nyssen et al., 2008a; Hurni et al., 2010), socio-political changes (Dessie and Christiansson, 2008), and unbalanced free grazing with the still-increasing livestock populations (Taddese, 2001; Abiyu et al., 2011). Intensified use of land and dwindling of the remnant forests in central Ethiopia are further exacerbating land degradation (Abebe et al., 2014).

In Ethiopia, land degradation impairs the capacity of forests, to provide ecosystem services (Solomon et al., 2018) and the land to contribute to food security (Bishaw, 2001). It also results in significant environmental degradation and loss of forest biodiversity (Lemenih and Kassa, 2014). Estimates show that the country is losing up to 1636 km2 yr-1 forest cover, which is one of the highest in Africa (Tolera et al., 2008). Land degradation in Ethiopia has not only resulted in biomass loss, but also an increase in risks of desertification (Girmay et al., 2008). Further deforestation contributed to climate change by causing changes in the earth’s atmosphere in the amounts of greenhouse gases. Land resources degradation in Ethiopia resulted in erosion induced soil carbon depletion rate of up to 970 kg ha yr-1 (Shiferaw et al., 2013). Land degradation increases the vulnerability of people to the adverse effects of climate variability and change. As a result of unfavorable climate conditions, poor agricultural technologies, and high population growth, the magnitude of land degradation is more severe in arid regions of Ethiopia (Meaza et al., 2017).

The ecological restoration, which is the process of assisting the rehabilitation of degraded ecosystems (Ruiz-Jaen and Aide, 2005) has been an important approach to mitigate human pressures on natural ecosystems and improve ecosystem services (Mekuria et al., 2015). To rehabilitate the vast degraded landscapes and combat environmental problems, area exclosures have commenced since the 1980s in the northern highlands of Ethiopia (Aerts et al., 2009). Exclosures are areas that are excluded from the disturbance of domestic animals and humans to promote the natural regeneration of plants and reduce land degradation (Aerts et al., 2009). The ultimate goal of restoration using exclosure is to create a self-supporting ecosystem that is resilient to perturbation without further assistance (Ruiz-Jaen and Aide, 2005). Over the past two decades, exclosures have been expanded to the central part of the country (Mengistu et al., 2005; Yirdaw and Monge, 2018). Effective restoration of degraded ecosystems is one of the imperative efforts to ensure sustainable food and biomass supply to the heavily agricultural dependence of the population of the country while maintaining ecological integrity (Lemenih, 2006).

Restoration of degraded lands through the establishment of exclosures is crucial to support people and food systems as well as to adapt to climate variability (Mekuria et al., 2017). Studies have indicated that after exclosure was established on degraded landscape vegetation restored (Birhane et al., 2006; Mekuria and Yami, 2013; Mekuria et al., 2015; Ibrahim, 2016). Exclosures can enhance soil nutrients such as total soil nitrogen, available phosphorus, and cation exchange capacity (Descheemaeker et al., 2006b; Mekuria and Aynekulu, 2013; Abebe et al., 2014). Furthermore, exclosure can improve soil organic carbon (Yimer et al., 2015; Qasim et al., 2017) and increase the potential of atmospheric carbon sequestration (Feyisa et al., 2017). Area exclosures are likely to improve soil carbon stock thereby increasing the amount and quality of the biomass that is recycled into the soil (Chen et al., 2012). Restoration of vegetation in severely degraded lands in dry regions has a considerable potential to increase carbon storage while improving soil quality (Li et al., 2012).

Exclosure land management began to be practiced fifteen years ago to restore the degraded landscapes in Kewet district, which is part of the central dry lowlands of Ethiopia (Bizuayehu and Tefera, 2008). Unlike exclosures on other parts of the country, less attention has been given to exclosure in the central dry lowland of Ethiopia in the assessment of their effectiveness in the restoration of vegetation. Little is known about aboveground biomass and carbon stock of exclosures in response to their age (Giday et al., 2013). Earlier most studies conducted on area exclosures in Ethiopia focused on the comparison of vegetation composition and soil properties with adjacent grazing lands (Abiyu et al., 2011; Mekuria et al., 2018; Haftom et al., 2019). Except for very few (Descheemaeker et al., 2006a; Mekuria and Yami, 2013), change in vegetation composition along chronosequence is not yet well studied. Despite the number of studies, most studies lack integrating climate change issues i.e., determination of carbon stock. This helps the management and policy decision-makers related to the expansion of such land use management to restore degraded lands of the lowland areas. Therefore, this study aimed to quantify 1) the vegetation structure and diversity and 2) aboveground biomass and carbon stock of different aged exclosures and adjacent open grazing land in the central dry lowland of Ethiopia.

2. MATERIALS AND METHODS

2.1. Description of the Study Area

The study was conducted in Kewet district situated 213 km northeast of Addis Ababa at the foot of the eastern escarpment of the central Ethiopian highlands. It is located within the coordinates of 9°49′ and the 10°11' latitude N and 39°45′ to 40°6′ longitude E. Administratively, Kewet district lies within the North Shewa Administrative Zone of the Amhara National Regional State (Fig. 1). The altitude ranges from 1080 to 3151 m a.s.l. Based on 10 years (2007–2016) of data from the Shewa Robit meteorology station, the area receives a mean annual rainfall of 1007 mm and a mean annual minimum and maximum temperature of 16.5 and 31°C, respectively. Kewet district lay in Robit Valley which is widely covered by basalt and related pyroclastics rock types of tertiary age (Tefera et al., 1996). According to World Reference Base (WRB), Eutric Cambislos and pellic vertisols are the dominant soil groups in the alluvial fan area of the district lies the lower areas are dominantly covered with Calcaric gleysols and Calcic cambisols (Paris, 1986). Over 41 and 26% of the district’s land is covered by cultivated land, and forest and shrubland, respectively (Tesfay et al., 2020). Area exclosure practice is common in the district, which covers 15 km2 of the total land coverage (Bizuayehu and Tefera, 2008). According to Ethiopia’s Central Statistical Agency (CSA) population projection for all regions at wereda level from 2014 – 2017, the district has a total population of 147,093 with a population density of 197 persons per km2 (CSA, 2013).

2.2. Sampling Technique and Sampling Size

Three exclosure sites which are 5, 15, and >20 years old and one adjacent open grazing land were selected purposively which hereafter is referred to as young, middle, and old age exclosure, respectively. Then, three parallel transect lines (which were considered as replicates) across the selected exclosures and open grazing land were laid. Quadrats measuring 20 × 20 m (400 m2) were laid down along the parallel line transects at a regular distance (Kent, 2012). Data on woody species type, height, and diameter at breast height (dbh) of individuals with dbh > 2 cm were collected from quadrats along each line transects within each site. The total number of individuals of each wood species seedling and saplings < 1.5 m height was counted and recorded in each quadrat (Kent, 2012). Hypsometer and caliper were used to measure the height and dbh of the tree, respectively. A total of 36 sample plots was used to collect data on woody vegetation. The woody species were identified directly in the field using (Hedberg and Edwards, 1989; Edwards et al., 1995; Hedberg et al., 2003, 2006). Geographical information such as location, elevation, slope, and aspect of each plot was recorded using a geographical positioning system (GPS).

Fig. (1). Map of the study site.

2.3. Vegetation Data Analyses

2.3.1. Floristic, Diversity and Structure Analysis of Woody Species

The similarity in the floristic composition of woody species between the different age of exclosures and adjacent open grazing land was computed using Sorensen's measure of similarity (Magurran, 2004) as follows:

(1)

where, a = is the number of species common to both exclosures and adjacent open grazing land, b = is the number of species on exclosure 1 or adjacent open grazing land (site 1) only, and c = is the number of species on exclosure 1 or adjacent open grazing land (site 2) only. Often the coefficient is multiplied by 100 to give a percentage similarity figure. Species richness is a biologically appropriate measure of alpha (α) diversity and it refers to the number of species in a particular area (Newton, 2007). The diversity of woody species was determined by the Shannon-Wiener diversity (H') index. The index takes into account the species richness and the proportion of each woody species in all sampled quadrats (Teketay et al., 2018). Shannon-Wiener diversity was calculated using the following equation (Magurran, 2004):

(2)

where, H’ = is a Shannon-Wiener (H') diversity index, Pi= is the proportion of individuals of woody species found in the quadrats, S= is the number of woody species and ln= natural logarithm. Woody species evenness which is a measure of similarity of the abundances of the different woody species in the sampled exclosure and grazing land sites (Teketay et al., 2018) was determined using Shannon evenness measure (E). It was calculated using the following equation (Magurran, 2004; Kent, 2012):

(3)

The woody species structure was determined through quantitative analysis using parameters such as frequency, density (number of trees ha-1) and basal area (m2 ha-1). Frequency is the number of times a species is recorded in a given number of plots and it is expressed as the percentage of quadrats in which a particular species is present (Newton, 2007). Density is the number of individuals of a species per unit area (Kent, 2012). Basal area (BA) is the cross-sectional area of a tree estimated at breast height (1.3 m), which is expressed in m2. Basal area was calculated using the following equation (Newton, 2007):

(4)

where: g = is a basal area (m2), and d = is the diameter at breast height of a tree (m).

The importance value index (IVI) is used as a measure of species composition that combines frequency, abundance, and dominance values. The ecological importance of woody species (IVI) was determined using relative frequency, abundance, and dominance parameters according to the formula of (Kent, 2012):

(5)
(6)
(7)
(8)

where, *Dominance is defined as the mean basal area per tree, multiplied by the number of trees of the species.

2.3.2. Aboveground Woody Biomass and Carbon Stocks Estimation

To estimate the aboveground woody biomass (AGB) in the exclosures and adjacent open grazing land, an allometric equation developed (Tetemke et al., 2019) for multispecies of the dry Afromontane forests in dry mid-highlands of Northern Ethiopia was used.

(9)

where, AGB = Aboveground biomass (kg tree-1) dbh = diameter at breast height (cm) rRMSE = relative root mean square error, and rBias = relative bias

Belowground biomass is often estimated from root: shoot ratios by taking 28% of the aboveground biomass ((Intergovernmental Panel on Climate Change (IPCC) et al., 2006;) and it was estimated as follows:

(10)

where, AGB = Aboveground biomass (kg tree-1)

Then both the above and belowground biomass were converted into carbon by multiplying the aboveground (or belowground) biomass by 0.47 (IPCC, 2006):

(11)

where, Y = wood biomass-1 (kg)

2.4. Statistical Data Analysis

Data on vegetation structure, composition, aboveground biomass, and aboveground biomass carbon stock were subjected to a one-way analysis of variance (ANOVA) following a generalized linear model (GLM) of SAS 9.2 software. Post Hoc Test of Tukey’s Honest Significant Difference (HSD) test was used for mean separation if the analysis of variance showed statistically significant differences (p < 0.05).

3. RESULTS AND DISCUSSION

3.1. Floristic Composition

A total of 21 species that represent 9 families were recorded both in the exclosures and adjacent open grazing land. Woody species richness ranged from 10 species in the open grazing land to 14, 15, and 17 species in the middle, old, and young age exclosures, respectively. This result is comparable to similar studies conducted by Haftom et al., (2019) in Tigray, Northern Ethiopia, and Abiyu et al., (2011) in South Wollo, Northern Ethiopia. which reported 17 and 14 woody species, respectively in area exclosures. However, the result of this study is lower when compared to reports of Mekuria et al., (2018) which reported 32 woody species in exclosures in the North-Western Ethiopia and Teketay et al., (2018) which recorded 35 woody species in an exclosure in the Mopane woodland in northern Botswana.

Analysis of the Sorensen’s similarity index showed that between all sites there was over 50 and 40% similarity of woody species and family, respectively (Table 1). The woody species in the open grazing land were less similar to the woody species in the area exclosures, whereas the similarity of woody species between exclosure was higher than with the open grazing land. Of the total woody species encountered in the study area, six were common in all the exclosures and open grazing land and 11 species were present only in the exclosures. One woody species (Prosopis juliflora) was only recorded in the open grazing land and three woody species (Balanites aegyptiaca, Berchemia discolor, and Grewia ferruginea) were recorded only in the young age exclosure. The existence of Prosopis juliflora indicated that there is an encroachment of exotic and invasive tree species on the degraded open grazing land. Reports have indicated that certain woodlands in the Eastern Ethiopian highlands are causing some socio-cultural damage (FAO, 2015). In the study area and the nearby Afar region, the encroachment of Prosopis juliflora is becoming a great concern to the communal rangeland.

Table 1. Sorensen’s similarity index of woody species and family of exclosures and open grazing land.
Land use Species similarity (%) Family similarity (%)
OGL Yo-ex Mi-ex Ol-ex OGL Yo-ex Mi-ex Ol-ex
Open grazing land 100.00 51.85 58.33 58.33 100.00 40.00 100.00 100.00
Young age exclosure - 100.00 75.00 77.42 - 100.00 71.43 80.00
Middle age exclosure - - 100.00 93.33 - - 100.00 93.33
Old age exclosure - - - 100.00 - - - 100.00
OGL= open grazing land, Yo-ex = young age exclosure, Mi-ex = middle age exclosure, and Ol-ex = old age exclosure.

Of the total family recorded in the study area, Fabaceae was observed in all the exclosures and open grazing land, and Olacaceae was only recorded in the young age exclosures. Only three family names, i.e., Boraginaceae, Fabaceae, and Tiliaceae were observed in the open grazing land (Tables 4-7). As in many other places of Ethiopia, the Fabaceae family contributed the greatest number of species in the study area (Mekuria and Yami, 2013; Mekuria et al., 2015; Gebremedihin et al., 2018). Despite its consistency, both family numbers and species numbers increase with an increase in age of exclosure.

Table 2. Woody species Shannon index diversity and Shannon evenness.
Land Use Species Richness Shannon Index (H’) Shannon Evenness (E’)
Open grazing land 10 0.74 0.32
Young age exclosure 17 2.02 0.71
Middle age exclosure 14 2.12 0.80
Old age exclosure 15 2.06 0.76

3.2. Woody Species Diversity

The Shannon-Wiener (H') index ranged from 0.74 at the open grazing land to 2.12 at the middle age exclosure (Table 2). According to Magurran, (2004), the Shannon-Wiener (H')diversity index in the exclosures can be rated as moderate whereas the diversity in the open grazing land was very low. Usually, the value of the Shannon-Wiener diversity index ranged from 1.5 to 3.5 and rarely superpassed 4 for a very huge number of species (Magurran, 2004). Shannon-Wiener diversity index in the study area indicated that exclosures can improve the diversity of woody species in the degraded open grazing lands. The changes in species richness were also evident in the Shannon-Wiener. The mean value of the Shannon-Wiener index of this study is in line with other similar studies conducted by Mekuria et al., (2018) in West Gojam, North-Western Ethiopia, and Gebremedihin et al., (2018) in the highlands of Tigray, Northern Ethiopia.

The Shannon evenness (E) index was also lower (0.32) at the grazing land and it was higher (0.80) in the middle age exclosure (Table 2). The higher the evenness value, the more even the distribution of species is at a site (Kent, 2012). Thus, the result of this study showed that there was a better equitable distribution of individual woody species in the exclosure than open grazing land. The high Shannon evenness value in the middle age exclosure might be attributed to the low anthropogenic disturbance in the exclosures since it is excluded from unmanaged access. In line with this study Teketay et al., (2018) reported an evenly distributed individual of woody species in area exclosure than in the open grazing area in Mopane woodland in northern Botswana.

3.3. Woody Species Structure

3.3.1. Basal Area and Tree Density

As indicated in (Table 3), significantly (p < 0.05) higher woody species basal area was observed in the middle (20 m2 ha-1) and old age exclosure (20 m2 ha-1) than the young age exclosure (6 m2 ha-1) and open grazing land (3 m2 ha-1). This indicated an increase of volume and area occupied by woody species with exclosure age. The highest basal area in the exclosures could be due to the relatively long period of exclusion from interference which allowed woody species to grow bigger. Other similar studies conducted in North-Western Ethiopia by Mekuria et al., (2018) and in Eastern Tigray, Ethiopia by Birhane et al., (2007) reported higher basal area in area exclosures than adjacent open grazing lands. Furthermore, the mean woody species basal area of exclosures was higher than other similar studies conducted in different parts of Ethiopia (Mekuria et al., 2018; Haftom et al., 2019).

Table 3. Mean ± S.E of basal area and tree density of exclosures and open grazing land.
Land Use Basal Area (m2 ha-1) Tree Density (ha-1)
Open grazing land 3.54±1.75b 200.00±44.10b
Young age exclosure 6.29±1.54b 469.44±28.50a
Middle age exclosure 20.35±3.92a 483.33±26.02a
Old age exclosure 20.29±5.09a 559.33±81.86a
p-value < 0.001 < 0.001
CV% 81.26 35.28
Means±S.E. with different letters within a column are significantly different (p < 0.05) (Tukey’s test HSD).

Tree density showed a significant difference (p < 0.05) between exclosures and the open grazing lands. It was ranged from 200 trees ha-1 in the grazing land to 559 trees ha-1 in the old age exclosure (Table 3). Tree density showed an increasing trend with increasing age of the exclosure. It is observed in this study that woody species density was positively influenced by exclosures. The tree density in the old exclosure was two and a half times higher than the tree density in the open grazing land. Similar studies were conducted by Mekuria et al., (2018) in West Gojam, North-Western Ethiopia, and by Teketay et al., (2018) in Mopane woodland in northern Botswana which showed a higher woody species density in exclosure than their adjacent grazing land. However, compared to these studies, the mean tree density in the study area was very small. This might attribute to differences in the management practice of the exclosures.

Table 4. Tree density (ha-1), RF (%), RA (%), RD (%) and IVI (%) of woody species of open grazing land.
Species Names Family TD RF RA RD IVI
Acacia tortilis (Forssk.) Hayne. Fabaceae 53.13 17.24 22.37 34.35 73.96
Acacia nilotica (L.) Willd. Ex Del. Fabaceae 37.5 6.9 15.79 24.44 47.12
Acacia etbaica Schweinf. Fabaceae 43.75 13.79 18.42 11.22 43.43
Acacia senegal (L.) Willd. Fabaceae 15.63 13.79 6.58 15.05 35.43
Dichrostachys cinerea (L.) Wight & Am. Fabaceae 28.13 10.34 11.84 2.9 25.09
Ehretia cymosa Thonn. Boraginaceae 15.63 10.34 6.58 3.23 20.15
Acacia seyal Del. Fabaceae 12.5 6.9 5.26 7.48 19.64
Grewia villosa Willd. Tiliaceae 15.63 10.34 6.58 0.47 17.39
Acacia brevispica Harms. Fabaceae 12.5 6.9 5.26 0.54 12.7
Prosopis juliflora (Sw.) DC. Fabaceae 3.13 3.45 1.32 0.33 5.09
- - - 100 100 100 300
TD = Trees density, RF = Relative frequency, RA = Relative abundant, RD = Relative dominance and IVI = Important Value Index.

3.3.2. Important Value Index

Analysis of IVI species in the open grazing land showed that Acacia tortilis (74%), Acacia nilotica (47%), and Acacia etbaica (43%) were the most important valued species (Table 4). In the young age exclosure Acacia brevispica (82%), Acacia tortilis (58%), and Dichrostachys cinerea (24%) were woody with high IVI value (Table 5). Acacia senegal (67%), Acacia brevispica (61%), and Acacia nilotica (58%) in the middle age exclosure (Table 6) and Acacia senegal (99%), Acacia nilotica (57%), and Acacia brevispica (36%) in old exclosure (Table 7) were the woody species which had high IVI value. Analysis of IVI in the study area showed that Acacia senegal was one of the woody species which make the top five IVI species across all the exclosures and the open grazing land. In addition to this, Acacia brevispica is one of the species which makes the top five IVI species in all the exclosures.

The IVI result has also shown that Acacia tree species were the most ecologically important species in both grazed and exclosures. The importance value index indicated the relative ecological importance of the woody species in the study area (Kent, 2012). The dry lowland area of Ethiopia is known for its dominance by Acacia tree species (Mengistu et al., 2005; Nyssen et al., 2008b; Birhane et al., 2010; Yimer et al., 2015). Feyisa et al., (2017) in Borana rangelands, Southern Ethiopia has reported that Acacia tortilis, Acacia seyal, and Balanites aegyptiaca dominated exclosure. Acacia species are among the common woody plants that naturally grow in many dry rangelands in sub-Saharan Africa (Yayneshet et al., 2008). These species are important litter producers in the exclosures established on degraded lands (Descheemaeker et al., 2006a) and subsequently, improve soil fertility (N2-fixing) and increase SOC stock (Nair et al., 2009; Abaker et al., 2016).

Table 5. Tree density (ha-1), RF (%), RA (%), RD (%) and IVI (%) of woody species of young age exclosure.
Species Names Family TD RF RA RD IVI
Acacia brevispica Harms. Fabaceae 205.56 4.55 43.79 34.5 82.83
Acacia tortilis (Forssk.) Hayne Fabaceae 41.67 15.91 8.88 32.73 57.52
Dichrostachys cinerea (L.) Wight & Am. Fabaceae 38.89 13.64 8.28 2.07 23.99
Acacia senegal (L.) Willd. Fabaceae 13.89 9.09 2.96 11.83 23.88
Euphorbia cactus Boiss. Euphorbiaceae 55.56 9.09 11.83 0.65 21.57
Delonix regia (Boj. ex Hook.) Raf. Fabaceae 16.67 9.09 3.55 3.58 16.22
Acacia nilotica (L.) Willd. Ex Del. Fabaceae 13.89 4.55 2.96 8.66 16.16
Euclea divinomm Murr Ebenaceae 27.78 6.82 5.92 0.2 12.94
Grewia villosa Willd. Tiliaceae 11.11 6.82 2.37 0.17 9.36
Grewia bicolor Juss. Tiliaceae 13.89 4.55 2.96 0 7.51
Balanites aegyptiaca (L.) Del. Balanitaceae 2.78 2.27 0.59 3.52 6.39
Berchemia discolor (Kiotzsch) Hemsl. Rhamnaceae 11.11 2.27 2.37 1.35 5.99
Grewia ferruginea Hochst. ex A. Rich. Tiliaceae 5.56 2.27 1.18 0.02 3.48
Acacia oerfota (Forssk.) Schweinf. Fabaceae 2.78 2.27 0.59 0.56 3.43
Ziziphus spina-ehristi (L.) Des. Rhamnaceae 2.78 2.27 0.59 0.1 2.96
Ximenia americana L. Olacaceae 2.78 2.27 0.59 0.04 2.9
Acacia seyal Del. Fabaceae 2.78 2.27 0.59 0 2.87
- - - 100 100 100 300
TD = Tree density, RF = Relative frequency, RA = Relative abundant, RD = Relative dominance and IVI = Importance Value Index.


Table 6. Tree density (ha-1), RF (%), RA (%), RD (%) and IVI (%) of woody species of middle-aged exclosure.
Species names Family TD RF RA RD IVI
Acacia senegal (L.) Willd. Fabaceae 113.9 12.5 17.83 36.27 66.59
Acacia brevispica Harms. Fabaceae 197.2 12.5 30.87 17.41 60.78
Acacia nilotica (L.) Willd. Ex Del. Fabaceae 80.56 12.5 12.61 32.66 57.77
Delonix regia (Boj. ex Hook.) Raf. Fabaceae 47.22 9.72 7.39 5.61 22.73
Euphorbia cactus Boiss. Euphorbiaceae 61.11 9.72 9.57 1.97 21.25
Grewia bicolor Juss. Tiliaceae 27.78 6.94 4.35 1.56 12.85
Ehretia cymosa Thonn. Boraginaceae 16.67 6.94 2.61 2.06 11.61
Grewia villosa Willd. Tiliaceae 25 5.56 3.91 1.24 10.71
Dichrostachys cinerea (L.) Wight & Am. Fabaceae 16.67 5.56 2.61 0.02 8.18
Euclea divinomm Murr Ebenaceae 22.22 4.17 3.48 0.02 7.66
Apodytes dimidiata E. Mey. Ex Am. Icacinaceae 8.33 4.17 1.3 0.51 5.98
Acacia etbaica Schweinf. Fabaceae 8.33 4.17 1.3 0.01 5.48
Acacia tortilis (Forssk.) Hayne Fabaceae 8.33 2.78 1.3 0.67 4.75
Acacia oerfota (Forssk.) Schweinf. Fabaceae 2.78 1.39 0.43 0 1.83
Ximenia americana L. Olacaceae 2.78 1.39 0.43 0 1.83
- - - 100 100 100 300
TD = Tree density, RF = Relative frequency, RA = Relative abundant, RD= Relative dominance and IVI = Importance Value Index.
Table 7. Tree density (ha-1), RF (%), RA (%), RD (%) and IVI (%) of woody species of old age exclosure.
Species names Family TD RF RA RD IVI
Acacia senegal (L.) Willd. Fabaceae 181.3 17.78 32.4 49.18 99.36
Acacia nilotica (L.) Willd. Ex Del. Fabaceae 78.13 13.33 13.97 29.21 56.51
Acacia brevispica Harms. Fabaceae 106.3 13.33 18.99 4.09 36.42
Acacia tortilis (Forssk.) Hayne Fabaceae 18.75 8.89 3.35 9.71 21.96
Acacia etbaica Schweinf. Fabaceae 21.88 8.89 3.91 6.69 19.49
Euphorbia cactus Boiss. Euphorbiaceae 53.13 8.89 9.5 0.11 18.5
Apodytes dimidiata E. Mey. Ex Am. Icacinaceae 28.13 6.67 5.03 0.05 11.74
Dichrostachys cinerea (L.) Wight & Am. Fabaceae 21.88 4.44 3.91 0.51 8.86
Delonix regia (Boj. ex Hook.) Raf. Fabaceae 15.63 4.44 2.79 0.08 7.32
Ximenia americana L. Olacaceae 15.63 2.22 2.79 0.21 5.23
Euclea divinomm Murr Ebenaceae 6.25 2.22 1.12 0.01 3.35
Ziziphus spina-ehristi (L.) Des. Rhamnaceae 3.13 2.22 0.56 0.12 2.9
Ehretia cymosa Thonn. Boraginaceae 3.13 2.22 0.56 0.01 2.79
Grewia bicolor Juss. Tiliaceae 3.13 2.22 0.56 0.01 2.79
Grewia villosa Willd. Tiliaceae 3.13 2.22 0.56 0 2.79
- - - 100 100 100 300
TD = Tree density, RF = Relative frequency, RA = Relative abundant, RD = Relative dominance and IVI = Importance Value Index.

3.3.3. Population Structure

The majority of the woody species in the study area had a height of below 5 m. The mean height of woody species was higher in the area exclosures than the open grazing land. Relatively the middle age exclosure and open grazing land have a higher number of woody species individuals > 5 m height (Figs. 2 and 4). The mean height of the species was 2.96±0.14, 2.37±0.08, 2.23±0.09, and 2.24±0.09 m for open grazing land, young, middle and old age exclosure, respectively, with the maximum height observed for Acacia brevispica and Acacia senegal (6.2 m) and the minimum for Apodytes dimidiate (0.2 m).

Diameter distribution of the community of all woody species in the study area showed an inverted J shape (Fig. 3). In open grazing land, most of the woody species were in the middle diameter classes (11-20 cm) and less in the lower diameter classes. The mean diameter of individual woody species was 16.91±0.99, 9.30±0.71, 14.62±0.98, and 14.80±1.17 cm for open grazing land, young, middle, and old exclosure, respectively. The dominance of remnant trees which are old, large and taller trees and less scattered existence of saplings and younger trees in the open grazing land might be the reason for the higher mean diameter of individual trees in the open grazing land than the exclosure. The maximum diameter was observed for Acacia senegal (80 cm) and the minimum for Dichrostachys cinerea, Grewia villosa, Ximenia americana, and Acacia brevispica (3 cm).

Both the height and diameter distribution graphs (Figs. 2 and 3) of this study depict that the open grazing land was dominated by shrubs and trees while exclosures had a higher number of tree seedlings and saplings. Thus, exclusion of disturbance favored the higher number of seedlings and saplings. Furthermore, the inverted J shape distribution of woody species dbh in the exclosures indicated that exclosure created favorable conditions for an active and uniform regeneration of woody species in the degraded and open grazing land. The higher mean height and diameters of woody species in grazing land might be due to seedlings and saplings exposed to domestic animals grazing and trampling. Due to exclusion from interference, there is a high probability of growing to the next diameter class in exclosures, that gives a better population structure. Similar investigations conducted in the highlands of Tigray, Northern Ethiopia by Negussie et al., (2008), and Birhane et al., (2017) have reported a higher abundance of tree seedling than adjacent open grazing lands. Other study by Teketay et al., (2018) also found higher seedling density in area exclosure than the adjacent open grazing system in Mopane woodland in northern Botswana.

Fig. (2). Woody species height distribution in exclosures and open grazing land.

Fig. (3). Woody species diameter distributions in exclosures and open grazing land.

Fig. (4). Partial view of a) open grazing land, b) young age exclosure, c) middle age exclosure, and d) old age exclosure in the central dry lowland of Ethiopia.

3.4. Aboveground Woody Biomass and Carbon Stock

Aboveground biomass and belowground biomass showed statistically significant (p < 0.05) variation between exclosures and open grazing land (Table 8). Aboveground biomass ranged from 12.60 Mg ha-1 at the open grazing land to 86.61 Mg ha-1 in the middle age exclosure. Aboveground biomass quantified in this study mirrored the basa area which is significantly higher in the middle and old age exclosures. This might explain the higher AGB in the middle and old age exclosures. In a study undertaken Mekuria et al., (2018) in North-Western Ethiopia, it was indicated that exclosure land management can improve soil properties, which consequently supports the restoration of native vegetation and the accumulation of aboveground biomass. The result of this study is in line with findings of Yayneshet, (2011) in the Tigray region of northern Ethiopia and Mekuria and Yami, (2013) in the lowlands of Northern Ethiopia which reported higher aboveground biomass in exclosures than adjacent grazing lands.


Table 8. Mean± S.E values of AGB (Mg ha-1), BGB (Mg ha-1), AGB-C stock (Mg ha-1) and BGB-C stock (Mg ha-1).
Land Use AGB BGB AGB-C BGB-C
Open grazing land 12.60±6.20b 3.15±1.55b 5.92±2.91b 1.66±0.82b
Young age exclosure 22.81±5.26b 5.70±1.31b 10.72±2.47b 3.00±0.69b
Middle age exclosure 68.61±12.47a 17.15±3.12a 32.25±5.86a 9.03±1.64a
Old age exclosure 67.85±15.96a 16.96±3.99a 31.89±7.50a 8.93±2.10a
p-value < 0.001 < 0.001 < 0.001 < 0.001
CV% 76.21 76.21 76.21 76.2
Means ±S.E. with different letters within a column are significantly different (p < 0.05) (Tukey’s test HSD).
AGB = Aboveground biomass, BGB = Belowground biomass, and C = Carbon.

The AGB carbon stock was significantly (p < 0.05) higher in the middle (32 Mg ha-1) and old (31 Mg ha-1) age exclosures whereas it was significantly lower in the open grazing land and young age exclosure. The BGB carbon stock followed a similar trend to the AGB carbon stock. The highest AGB carbon stock in the middle and old age exclosure could be associated with the high tree density and tree basal area. Except in the young exclosure, AGB and carbon stock had shown consistent increase from middle age to old exclosure age (Table 8). In line with this, several studies have indicated a significantly higher AGB carbon stock in exclosure than adjacent open grazing lands. For instance, studies conducted by Shimelse et al., (2017) in Tigray and Bikila et al., (2016) in Borana, North and Southern Ethiopia, have reported a significantly higher AGB carbon stock in exclosures than open grazing land. Another study by Mekuria and Yami, (2013) also displayed higher AGB in exclosures when compared to the adjacent communal grazing lands.

More importantly, this study demonstrated that pronounced woody biomass carbon can be stocked in the long-term. Generally, excluding degraded landscapes from free access of domestic animals and humans might bring an overall ecological restoration to the sites. Thus, such a restoration mechanism might contribute to the efforts of mitigating climate change in developing counties. A study by Witt et al., (2011) indicated a pronounced woody biomass carbon stock and significant ecological change following long-term grazing exclusion.

CONCLUSION

This study evaluated the influence of different ages of exclosure and adjacent open grazing land on woody vegetation structure, composition, and aboveground biomass carbon stock in the central dry lowland of Ethiopia. The results demonstrate that a considerable amount of woody species was restored due to the exclusion of open grazing land for rehabilitation purposes. Furthermore, woody species structure i.e., basal area, tree density, and composition were significantly influenced by age of exclosures. Excluding grazing animals and human interferences from accessing severely degraded lands for long period resulted in an approximate doubling of the tree density, standing basal area, species diversity, aboveground biomass, and carbon stock relative to grazed areas. The significantly higher aboveground biomass and aboveground biomass carbon stock clearly showed that degraded land restoration strategies through exclosure can sequester a large quantity of atmospheric carbon dioxide. To comprehend more on the impact of area exclosures on carbon stock, further investigation on belowground biomass and soil carbon stock is suggested.

LIST OF ABBREVIATIONS

AGB  = Aboveground Biomass
BGB  = Belowground biomass
H'  = Shannon-Wiener diversity index
BA  = Basal Area
dbh  = Diameter at breast height
HSD  = Honest Significant Difference
IVI  = Importance Value Index
RA  = Relative Abundance
RD  = Relative Dominance
RF  = Relative Frequency

CONSENT FOR PUBLICATION

Not applicable.

AVAILABILITY OF DATA AND MATERIALS

Not applicable.

FUNDING

Not applicable.

CONFLICT OF INTEREST

The authors declare that they have no any conflict of interest.

ACKNOWLEDGEMENTS

Debre Birhan University, Ethiopia is duly acknowledged for the assistance of this study.

REFERENCES

Abaker WE, Berninger F, Saiz G, Braojos V, Starr M. Contribution of Acacia senegal to biomass and soil carbon in plantations of varying age in Sudan. For Ecol Manage 2016; 368: 71-80.
Elsevier BV, Abebe T, Feyssa DH, Kissi E. Area Exclosure as a Strategy to Restore Soil Fertility Status in Degraded Land in Southern Ethiopia. Journal of Biological and Chemical Research 2014; 31(1): 482-94.
Abiyu A, Lemenih M, Gratzer G, Aerts R, Teketay D, Glatzel G. Status of Native Woody Species Diversity and Soil Characteristics in an Exclosure and in Plantations of Eucalyptus globulus and Cupressus lusitanica in Northern Ethiopia. Mountain Research and Development 2014; 31(2): 144-52.
Aerts R, Nyssen J, Haile M. On the difference between “exclosures” and “enclosures” in ecology and the environment. Journal of Arid Environments 2011; 73(8): 762-3.
Appanah S, Shono K, Durst PB. Restoration of forests and degraded lands in Southeast Asia. Unasylva 66(245): 52-63.
Berisso T. Deforestation and Environmental Degradation in Ethiopia: The Case of Jam Jam Province. Northeast African Studies 1995; 2(2 (New Series)): 139-55.
Bikila NG, Tessema ZK, Abule EG. Carbon sequestration potentials of semi-arid rangelands under traditional management practices in Borana, Southern Ethiopia. Agriculture, Ecosystems and Environment 2016; 223(2 (New Series)): 108-14.
Birhane E, Aregawi K, Giday K. Changes in arbuscular mycorrhiza fungi spore density and root colonization of woody plants in response to exclosure age and slope position in the highlands of Tigray, Northern Ethiopia. J Arid Environ 2017; 142: 1-10. [Elsevier Ltd.].
Birhane E, Kuyper TW, Sterck FJ, Bongers F. Arbuscular mycorrhizal associations in Boswellia papyrifera (frankincense-tree) dominated dry deciduous woodlands of Northern Ethiopia. For Ecol Manage 2010; 260(12): 2160-9. [Elsevier B.V.].
Birhane E, Teketay D, Barklund P. Actual and potential contribution of exclosures to enhance biodiversity of woody species in the drylands of Eastern Tigray Actual and potential contribution of exclosures to enhance biodiversity of woody species in the drylands of Eastern Tigray.. Journal of the Drylands 2006; 1(2): 134-47.
Birhane E, Teketay D, Barklund P. East African Journal of Sciences (2007) Enclosures to Enhance Woody Species Diversity in The Dry Lands of Eastern Tigray. East African Journal of Sciences 2007; 1: 136-47.
Bishaw B. Deforestation and Land Degredation in the Ethiopian Highlands: A Strategy for Physical Recovery. Northeast Afr Stud 2001; 8(1): 7-25.
Bizuayehu S, Tefera B. Assessment on Socioeconomics Aspects of Area Enclosures in North Shewa Zone: The case of Kewote and Basona Worena woredas 2008; 1-15.
Chen Y, Li Y, Zhao X, Awada T, Shang W, Han J. Effects of grazing exclusion on soil properties and on ecosystem carbon and nitrogen storage in a sandy rangeland of Inner Mongolia, northern China. Environ Manage 2012; 50(4): 622-32.
CSA (Central Statistical Agency Ethiopia). Population Projection of Ethiopia for All Regions At Wereda Level from 2014 – 2017 Federal Democratic Republic of Ethiopia Central Statistical Agency. 2013.
Descheemaeker K, Muys B, Nyssen J, et al. Litter production and organic matter accumulation in exclosures of the Tigray highlands, Ethiopia. For Ecol Manage 2006; 233(1): 21-35. a
Descheemaeker K, Nyssen J, Poesen J, et al. Soil and Water Conservation Through Forest Restoration in Exclosures of the Tigray Highlands. Journal of the Drylands 2006; 1(2): 118-33. b
Dessie G, Christiansson C. Forest decline and its causes in the south-central rift valley of Ethiopia: human impact over a one hundred year perspective. Ambio 2008; 37(4): 263-71.
Edwards S, Tadesse M, Hedberg I, Eds. Flora of Ethiopia and Eritrea:Canellaceae to Euphorbiaceae The National Herbarium. Addis Ababa University 1995.
Etter H. Land Degradation: An Economic Perspective. Land Restoration Elsevier Inc. 2016.
FAO. Global Forest Resources Assessment (FRA)-Country Report. Ethiopia. Rome, Italy 2015.
Feyisa K, Beyene S, Angassa A, Said MY, De Leeuw J. Effects of enclosure management on carbon sequestration, soil properties and vegetation attributes in East African rangelands. Catena 2017; 159: 9-19. [Elsevier.].
Gebremedihin KM, Birhane E, Tadesse T, Gbrewahid H. Restoration of Degraded Drylands Through Exclosures Enhancing Woody Species Diversity and Soil Nutrients in the Highlands of Tigray, Northern Ethiopia. Nature Conservation Research 2018; 3(1): 1-20.
Giday K, Eshete G, Barklund P, Aertsen W, Muys B. Wood biomass functions for Acacia abyssinica trees and shrubs and implications for provision of ecosystem services in a community managed exclosure in Tigray, Ethiopia. J Arid Environ 2013; 94: 80-6. [Elsevier Ltd.].
Girmay G, Singh BR, Mitiku H, Borresen T, Lal R. Carbon stocks in Ethiopian soils in relation to land use and soil management. Land Degrad Dev 2008; 19(4): 351-67.
Haftom H, Girmay T, Emiru B, Haftu A, Meseret H. Impact of farm exclosure on woody species abundance and carbon stock in Tigray, Northern Ethiopia. Cogent Environ Sci 2019; 5(1): 1-10. [Cogent.].
Haile M, Herweg K, Stillhardt B. Sustainable Land Management – A New Approach to Soil and Water Conservation in Ethiopia. Mekelle University 2006.
Hedberg I, Edwards S, Eds. Flora of Ethiopia: Pittosporaceae to Araliaceae The National Herbarium. Addis Ababa University 1989.
Hedberg I, Edwards S, Nemomissa S, Eds. Flora of Ethiopia and Eritrea: Apiaceae to Dipsacaceae The National Herbarium. Addis Ababa University 2003.
Hedberg I, Kelbessa E, Edwards S, Demissew S, Persson E, Eds. Flora of Ethiopia and Eritrea: Gentianaceae to Cyclocheilaceae The National Herbarium, Biology DeThe National Herbarium. Addis Ababa University 2006.
Hurni H, Solomon A, Amare B, et al. Land degradation and sustainable land management in the highlands of Ethiopia.Global change and sustainable develoopment: a synthesis of regional experiences from research partnershipsPerspectives of the Swiss National Centre of Competence in Research (NCCR) North-South. University of Bern 2010; pp. 187-207.
Ibrahim MA. Impact of Enclosure on Plant Species Composition and Biomass Production in Ewa Woreda of Afar Region State, Ethiopia. Journal of Biodiversity & Endangered Species 2016; 4(1): 1-4.
Intergovernmental Panel on Climate Change (IPCC). IPCC Guidelines for National Greenhouse Gas Inventories The Intergovernmental Panel on Climate Change (IPCC) 2006.
Kent M. 2012.Vegetation Description and Data Analysis: A Practical Approach http://books.google.com/books?id=xHnXf0wHdgQC&pgis=1
Lemenih M. Expediting ecological restoration with the help of foster tree plantations in Ethiopia. journal of the drylands 2006; 1(1): 72-84.
Lemenih M, Kassa H. Re-greening Ethiopia: History, challenges and lessons. Forests 2014; 5(8): 1896-909.
Li Y, Zhou X, Brandle JR, Zhang T, Chen Y, Han J. Temporal progress in improving carbon and nitrogen storage by grazing exclosure practice in a degraded land area of China ’ s Horqin Sandy Grassland. Agric Ecosyst Environ 2012; 159: 55-61. [Elsevier B.V.].
Magurran AE. Measuring Biological Diversity. Blackwell Science Ltd. 2004.
Meaza H, Frankl A, Poesen J, et al. Natural resource opportunities and challenges for rural development in marginal grabens e The state of the art with implications for the Rift Valley system in Ethiopia. J Arid Environ 2017; 147: 1-16. [Elsevier Ltd.].
Mekuria W, Aynekulu E. Exclosure land management for restoration of the soils in degraded communal grazing lands in northern ethiopia. Land Degrad Dev 2013; 24(6): 528-38.
Mekuria W, Langan S, Johnston R, et al. Restoring aboveground carbon and biodiversity: A case study from the Nile basin, Ethiopia. Forest Sci Technol 2015; 11(2): 86-96.
Mekuria W, Langan S, Noble A, Johnston R. Soil Restoration after seven Years of Exclosure Management in Northwestern Ethiopia. Land Degrad Dev 2017; 28(4): 1287-97.
Mekuria W, Wondie M, Amare T, Wubet A, Feyisa T, Yitaferu B. Restoration of degraded landscapes for ecosystem services in North-Western Ethiopia. Heliyon 2018; 4(8)e00764
Mekuria W, Yami M. Changes in woody species composition following establishing exclosures on grazing lands in the lowlands of Northern Ethiopia. Afr J Environ Sci Technol 2013; 7(January): 30-40.
Mengistu T, Teketay D, Hulten H, Yemshaw Y. The role of enclosures in the recovery of woody vegetation in degraded dryland hillsides of central and northern Ethiopia. J Arid Environ 2005; 60(2): 259-81.
Nair PKR, Nair VD, Kumar BM, Haile SG. Soil carbon sequestration in tropical agroforestry systems : A feasibility appraisal §. Environ Sci Policy 2009; 12: 1099-111.
Negussie A, Aerts R, Gebrehiwot K, Muys B. Seedling mortality causes recruitment limitation of Boswellia papyrifera in northern Ethiopia 2008; 72: 378-83.
Newton A. Forest Ecology and Conservation: A Handbook of Techniques. Oxford University Press 2007.
Nyssen J, Frankl A, Zenebe A, Deckers J, Poesen J. Land Management in the Northern Ethiopian Highlands: Local and Global Perspectives; Past, Present and Future. Land Degrad Dev 2015; 26(7): 759-64.
Nyssen J, Naudts J, De Geyndt K, et al. Soils and land use in the Tigray Highlands (Northern Ethiopia). Land Degrad Dev 2008; 19(3): 257-74. a
Nyssen J, Temesgen H, Lemenih M, Zenebe A, Haregeweyn N, Haile M. Spatial and temporal variation of soil organic carbon stocks in a lake retreat area of the Ethiopian Rift Valley. 2008; 146: pp. 261-8.
Paris S. Reconnaissance Soil Survey and Land Evaluation for Irrigation Purposes of an area near Robit (Shewa). National Water Resource Commission, Water Resource Development Authority of Ethiopia 1986.
Qasim S, Gul S, Shah MH, et al. Influence of grazing exclosure on vegetation biomass and soil quality. International Soil and Water Conservation Research 2017; 5(1): 62-8. [Elsevier B.V.].
Ruiz-Jaen MC, Aide TM. Restoration success: How is it being measured? Restor Ecol 2005; 13(3): 569-77.
Shiferaw A, Hurni H, Zeleke G. Long-term changes in soil-based ecological services at three sites in Ethiopia 2013; 5(August): 172-80.
Shimelse S, Bekele T, Nemomissa S. Effect of Exclosure Age on Carbon Sequestration Potential of Restorations in Tigray Region, N. Ethiopia. American Journal of Biological and Environmental Statistics 2017; 3(4): 65-80.
Solomon N, Hishe H, Annang T, Pabi O, Asante IK, Birhane E. Forest cover change, key drivers and community perception in Wujig Mahgo Waren forest of northern Ethiopia. Land (Basel) 2018; 7(1)
Taddese G. Land degradation: A challenge to Ethiopia. Environ Manage 2001; 27(6): 815-24.
Tefera M, Chernet T, Haro W. Geological Map of Ethiopia: Topgraphic base map, scale 1:2,000,0002 2 ed Geological Survey of Ethiopia. 1996.
Teketay D, Kashe K, Madome J, et al. Enhancement of diversity, stand structure and regeneration of woody species through area exclosure: The case of a mopane woodland in northern Botswana. Ecol Process 2018; 7(1): 1-15. [Ecological Processes.].
Tesfay F, Kibret K, Gebrekirstos A, Hadigu KM. Changes in selected soil properties across a chronosequence of exclosures in the central dry lowlands of ethiopia. Eurasian J Soil Sci 2020; 9(2): 173-85.
Tetemke BA, Birhane E, Rannestad MM, Eid T. Allometric models for predicting aboveground biomass of trees in the dry afromontane forests of Northern Ethiopia. Forests 2019; 10(12): 1-15.
Tolera M, Asfaw Z, Lemenih M, Karltun E. Woody species diversity in a changing landscape in the south-central highlands of Ethiopia. Agric Ecosyst Environ 2008; 128(1–2): 52-8.
Witt GB, Noël MV, Bird MI, Beeton RJS, Menzies NW. Carbon sequestration and biodiversity restoration potential of semi-arid mulga lands of Australia interpreted from long-term grazing exclosures. Agric Ecosyst Environ 2011; 141(1–2): 108-18. [Elsevier B.V.].
Yayneshet T. Restoration of Degraded Semi-Arid Communal Grazing Land Vegetation Using the Exclosure Model. International Journal of Water Resources and Arid Environment 2011; 1(5): 382-6.
Yayneshet T, Eik LO, Moe SR. Feeding Acacia etbaica and Dichrostachys cinerea fruits to smallholder goats in northern Ethiopia improves their performance during the dry season. Livest Sci 2008; 119(1–3): 31-41. [Elsevier B.V.].
Yimer F, Alemu G, Abdelkadir A. Soil property variations in relation to exclosure and open grazing land use types in the Central Rift Valley area of Ethiopia. Environmental Systems Research 2015; 4(1)
Yirdaw E, Monge A. Reconsidering what enclosure and exclosure mean in restoration ecology 2018; 26(1): 45-7.